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Mixed lymphocyte reaction
Mixed Lymphocyte Reaction (MLR); Synonyms also mixed leukocyte reaction and mixed leukocyte culture (MLC)
==History & Background==
The mixed lymphocyte reaction (MLR) is an ex vivo cellular immune assay that occurs between two allogeneic lymphocyte populations (same species but genetically distinct).〔1. Meo, T.: The MLR test in the mouse. 1979. In Immunological Methods, Academic Press, Inc., New York, NY.〕 It was first recognized when researchers mixed leukocytes from two unrelated donors in culture.〔2. Bain, B., Vas, M. and L. Lowenstein. 1963. A reaction between leukocytes in mixed peripheral blood cultures. Fed. Proc. 22:4281.〕〔3. Bach, F.H. and K. Hirschhorn. 1964. Lymphocyte interaction: A potential histocompatibility test in vitro. Science 143:813.〕 After several days, lymphocytes underwent blast transformation, DNA synthesis and cellular proliferation in response to the major histocompatibility antigen (MHC Class I and II) differences between the two cell populations designated as Responder and Stimulator cells. Responder cells proliferated without previous exposure to Stimulator MHC antigens. This response became quantifiable when incorporating radioactivity, () thymidine or 5-bromo-2’-deoxyuridine (BrdU), into the mixed cell suspension. This cellular response to the histocompatibility antigens that occurs in the MLR is also involved in cell-mediated immune responses within an individual and offered an in vitro correlate of cellular immune function.〔4. Benacerraf, B. and H.O. McDevitt. 1972. Histocompatibility linked immune response genes. Science (Wash D.C.) 175: 273.〕〔5. Katz, D.H. and B. Benacerraf, editor. 1976. The Role of Products of the Histocompatibility Gene Complex in Immune Responses. Academic Press, Inc., New York.〕 Standard MLR assays were performed in humans and most other animal species.
The leukocyte subpopulations involved in the MLR were first characterized by using cells from neonatally thymectomized and bursectomized chickens. No MLR occurred when the Responder cells came from thymectomized animals, whereas bursectomized chicken leukocytes reacted in culture demonstrating that T-cells were the major cell type in Responder cell populations.〔6. Alm, G.V. and R.D. Peterson: Effect of thymectomy and bursectomy and the in vitro response of chick spleen cells to PHA sheep erythrocytes and allogeneic cells. Fed. Proc. 29:430, 1970.〕
Originally, this assay was used to study possible donor — recipient incompatibilities for graft transplants to help predict better outcomes.〔7. Bach, F.H. 1970. Transplantation: pairing of donor and recipient. Science 168; 1170.〕 However, the standard for graft matching now depends on a series of HLA-matching done with molecular typing methods.〔8. Olerup, O and H. Zetterquist. 1992. HLA-DR typing by PCR amplification with sequence-specific primers (PCR-SSP) in 2 hours: An alternative to serological DR typing in clinical practice including donor-recipient matching in cadaveric transplantation. Tissue Antigens. 39:225.〕
抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』
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